The research conducted by the Magli lab aims at understanding the molecular mechanisms at the basis of cell differentiation and stem cell maintenance, which play important roles during development and in tissue homeostasis. For these studies, Dr. Magli uses the skeletal myogenic lineage as model system. By taking advantage of in vitro and in vivo models, his goal is to define how regulatory elements within the genome contribute to the acquisition of specific cell properties and, potentially, help to develop better therapeutic strategies for the treatment of muscle disorders.
Current Lab Projects
Molecular mechanisms of diaphragm development and homeostasis.
This project focuses on the identification of a unique population of myogenic progenitors that contribute to muscularization of the mammalian diaphragm. Identification of regulatory elements driving skeletal myogenic development and homeostasis. Using epigenetic profiling, the goal of these studies is to understand how regulatory elements interact in the mammalian nucleus and contribute to gene regulation.
Full list of publications at Experts@Minnesota.
- Magli A#, Baik J, Pota P, Ortiz Cordero C, Kwak IY, Garry DJ, Love PE, Dynlacht BD and Perlingeiro RCR#.(2019) Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification. Nat Commun. 10(1):2316. PMID: 31127120. #co-corresponding authors
- Magli A#, Baik J, Mills LJ, Kwak IY, Dillon BS, Mondragon Gonzalez R, Stafford DA, Swanson SA, Stewart R, Thomson JA, Garry DJ, Dynlacht BD, Perlingeiro RCR#. (2019) Time-dependent Pax3-mediated chromatin remodeling and cooperation with Six4 and Tead2 specify the skeletal myogenic lineage in developing mesoderm. PLoS Biology. 17(2):e3000153. PMID:30807574. #co-corresponding authors
- Selvaraj S, Mondragon-Gonzalez R, Xu B, Magli A, Kim H, Lainé J, Kiley J, Mckee H, Rinaldi F, Aho J, Tabti N, Shen W, Perlingeiro RCR. (2019) Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes. Elife. Nov 11;8. PMID: 31710288
- Magli A, Incitti T, Kiley J, Swanson SA, Darabi R, Rinaldi F, Selvaraj S, Yamamoto A, Tolar J, Yuan C, Stewart R, Thomson JA and Perlingeiro RCR (2017). PAX7 Targets, CD54, Integrin α9β1 and SDC2, Allow Isolation of Human ES/iPS Cell-Derived Myogenic Progenitors. Cell Reports. 19(13):2867-2877. PMID: 28658631
- Lilja KC, Zhang N, Magli A, Gunduz V, Bowman CJ, Arpke RW, Darabi R, Kyba M, Perlingeiro R, Dynlacht BD (2017). Pax7 remodels the chromatin landscape in skeletal muscle stem cells. PLoS One. 12(4):e0176190. PMID: 28441415